Molecular cloning and transcriptional analysis of the alkyl hydroperoxide reductase genes from Pseudomonas putida KT2442.
نویسندگان
چکیده
منابع مشابه
Transcriptional organization of the Pseudomonas putida tol-oprL genes.
Proteins of the Tol system play a key role in the maintenance of outer membrane integrity and cell morphology in gram-negative bacteria. In Pseudomonas putida, the seven genes, orf1, tolQ, tolR, tolA, tolB, oprL, and orf2, which encode the proteins of this complex, are clustered in a 5.8-kb region of chromosomal DNA. Analysis of polar mutations, reverse transcriptase PCR assays, and transcripti...
متن کاملSalmonella typhimurium LT-2, Pseudomonas putida KT2442 and Rhizobium meliloti
mhpT Encodes an Active Transporter Involved in 1 3-(3-Hydroxyphenyl)propionate Catabolism by Escherichia coli K-12 2 3 Ying Xu, Bing Chen, Hongjun Chao and Ning-Yi Zhou 4 5 Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of 6 Virology, Chinese Academy of Sciences, Wuhan 430071, China 7 8 *Corresponding author: Ning-Yi Zhou. 9 Email: [email protected]. 10 ...
متن کاملMolecular Cloning and Analysis of Two Flowering Related Genes from Apple (Malus × domestica)
Apple (Malus×domestica Borkh.) is the fourth fruit in importance and Iran ranks fifth in apple production in the world. Longevity of juvenility in apple extends breeding cycles and makes its breeding a tough job. To alleviate this barrier via genetic engineering, the genes involved in flowering and floral development of apple and their function must be identified and characterized. Most of thes...
متن کاملTwo-Dimensional Gel Electrophoresis Analysis of the Response of Pseudomonas putida KT2442 to 2-Chlorophenol.
The effects of exposure of Pseudomonas putida KT2442 to 2-chlorophenol as a model for the chemical stress response were examined by two-dimensional polyacrylamide gel electrophoresis. Individual protein concentrations were determined at 45, 65, and 95 min following the addition of 2-chlorophenol at a concentration of 1.63 mM to exponentially growing cultures of P. putida KT2442 by silver staini...
متن کاملMolecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.
We cloned the structural genes for the individual subunits of the branched-chain keto acid dehydrogenase multienzyme complex on a 7.8-kilobase EcoRI-SstI restriction fragment of Pseudomonas putida chromosomal DNA by cloning into the broad-host-range vector pKT230. A direct selection system for growth on valine-isoleucine agar was achieved by complementation of P. putida branched-chain keto acid...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 2001
ISSN: 0022-1260,1349-8037
DOI: 10.2323/jgam.47.269